| Short Description : | Rabbit polyclonal Shc-Transforming Protein 4 (1-185) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SHC4 |
| Gene ID: | 399694 |
| Uniprot ID: | SHC4_HUMAN |
| Immunogen Region: | 1-185 |
| Immunogen Sequence: | MRERGQDSLAGLVLYVGLFG HPGMLHRAKYSRFRNESITS LDEGSSGGSVGNKGSPQPPH PALAPHLPTEDATLPSQESP TPLCTLIPRMASMKLANPAT LLSLKNFCLGTKEVPRLKLQ ESRDPGSSGPSSPETSLSRS GTAPPPQQDLVGHRATALTP DSCPLPGPGEPTLRSRQDRH FLQHL |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human SHC4 (NP_976224.3). |
| Tissue Specificity | Only expressed in melanomas. Weakly expressed in normal melanocytes and benign nevi. Highly expressed at the transition from radial growth phase to vertical growth phase and metastatic melanomas, when tumor cells acquire migratory competence and invasive potential. |
| Post Translational Modifications | Phosphorylated.the phosphorylation is enhanced by EGF. Phosphorylation at Tyr-424 is required for the interaction with GRB2. |
| Function | Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1. |
| Protein Name | Shc-Transforming Protein 4Rai-Like ProteinRalpShc-Transforming Protein DHshcdSrc Homology 2 Domain-Containing-Transforming Protein C4Sh2 Domain Protein C4 |
| Database Links | |
| Cellular Localisation | Postsynaptic Cell MembraneColocalized With Musk At The Neuromuscular Junction |
| Alternative Antibody Names | Anti-Shc-Transforming Protein 4 antibodyAnti-Rai-Like Protein antibodyAnti-Ralp antibodyAnti-Shc-Transforming Protein D antibodyAnti-Hshcd antibodyAnti-Src Homology 2 Domain-Containing-Transforming Protein C4 antibodyAnti-Sh2 Domain Protein C4 antibodyAnti-SHC4 antibodyAnti-SHCD antibodyAnti-UNQ6438 antibodyAnti-PRO21364 antibody |
Information sourced from Uniprot.org
