| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase SH3RF1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SH3RF1 |
| Gene ID: |
57630 |
| Uniprot ID: |
SH3R1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of POSH at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human POSH |
| Function | Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing it's dynamin-dependent and clathrin-independent endocytosis. Acts as a scaffold protein that coordinates with MAPK8IP1/JIP1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the differentiation of CD4(+) and CD8(+) T-cells and promotes T-helper 1 (Th1) cell differentiation. Regulates the activation of MAPK8/JNK1 and MAPK9/JNK2 in CD4(+) T-cells and the activation of MAPK8/JNK1 in CD8(+) T-cells. Plays a crucial role in the migration of neocortical neurons in the developing brain. Controls proper cortical neuronal migration and the formation of proximal cytoplasmic dilation in the leading process (PCDLP) in migratory neocortical neurons by regulating the proper localization of activated RAC1 and F-actin assembly. (Microbial infection) Plays an essential role in the targeting of HIV-1 Gag to the plasma membrane, this function is dependent on it's RING domain, and hence it's E3 ligase activity. |
| Protein Name | E3 Ubiquitin-Protein Ligase Sh3rf1Plenty Of Sh3sProtein PoshRing Finger Protein 142Ring-Type E3 Ubiquitin Transferase Sh3rf1Sh3 Domain-Containing Ring Finger Protein 1Sh3 Multiple Domains Protein 2 |
| Database Links | Reactome: R-HSA-9013424Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmPerinuclear RegionCell ProjectionLamellipodiumGolgi ApparatusTrans-Golgi NetworkColocalizesWith Akt2In LamellipodiaWith Herp1In Trans-Golgi Network |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Sh3rf1 antibodyAnti-Plenty Of Sh3s antibodyAnti-Protein Posh antibodyAnti-Ring Finger Protein 142 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Sh3rf1 antibodyAnti-Sh3 Domain-Containing Ring Finger Protein 1 antibodyAnti-Sh3 Multiple Domains Protein 2 antibodyAnti-SH3RF1 antibodyAnti-KIAA1494 antibodyAnti-POSH antibodyAnti-POSH1 antibodyAnti-RNF142 antibodyAnti-SH3MD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
