Anti-SGLT1 antibody (Internal) (STJ73831)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73831-100
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General Information

Short DescriptionGoat polyclonal antibody anti-SGLT1 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications.
ApplicationsPep-ELISA/IHC
HostGoat
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:32000.
WB: Preliminary testing showed a band at approx. 60kDa in Human Gall Bladder lysate and in Jurkat cell lysate after 1-3µg/ml antibody staining (calculated MW of 60.1kDa according to NP_001
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolSLC5A1
Gene ID6523
Uniprot IDSC5A1_HUMAN
Accession NumberNP_000334.1; NP_001243243.1
Immunogen RegionInternal
Immunogen SequenceKETIEIETQVPEKKK

Additional Info

Post Translational Modifications N-glycosylation is not necessary for the cotransporter function.
Function Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose or D-galactose at the plasma membrane, with a Na(+) to sugar coupling ratio of 2:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Has a primary role in the transport of dietary monosaccharides from enterocytes to blood. Responsible for the absorption of D-glucose or D-galactose across the apical brush-border membrane of enterocytes, whereas basolateral exit is provided by GLUT2. Additionally, functions as a D-glucose sensor in enteroendocrine cells, triggering the secretion of the incretins GCG and GIP that control food intake and energy homeostasis. Together with SGLT2, functions in reabsorption of D-glucose from glomerular filtrate, playing a nonredundant role in the S3 segment of the proximal tubules. Transports D-glucose into endometrial epithelial cells, controlling glycogen synthesis and nutritional support for the embryo as well as the decidual transformation of endometrium prior to conception. Acts as a water channel enabling passive water transport across the plasma membrane in response to the osmotic gradient created upon sugar and Na(+) uptake. Has high water conductivity, comparable to aquaporins, and therefore is expected to play an important role in transepithelial water permeability, especially in the small intestine.
Protein Name Sodium/Glucose Cotransporter 1
Na(+/Glucose Cotransporter 1
High Affinity Sodium-Glucose Cotransporter
Solute Carrier Family 5 Member 1
Database Links Reactome: R-HSA-189200
Reactome: R-HSA-5656364
Reactome: R-HSA-8981373
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sodium/Glucose Cotransporter 1 antibody
Anti-Na(+/Glucose Cotransporter 1 antibody
Anti-High Affinity Sodium-Glucose Cotransporter antibody
Anti-Solute Carrier Family 5 Member 1 antibody
Anti-SLC5A1 antibody
Anti-NAGT antibody
Anti-SGLT1 antibody

Information sourced from Uniprot.org

Citations

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