• Immunohistochemistry analysis of paraffin-embedded rat heart using Delta-sarcoglycan (Delta-sarcoglycan (SGCD) ) antibody (STJ29060) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Delta-sarcoglycan (Delta-sarcoglycan (SGCD) ) antibody (STJ29060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SGCD antibody (61-290) (STJ29060)

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STJ29060

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SGCD (61-290) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGCD
Gene ID: 6444
Uniprot ID: SGCD_HUMAN
Immunogen Region: 61-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 61-290 of human Delta-sarcoglycan (Delta-sarcoglycan (SGCD) ) (NP_000328.2).
Immunogen Sequence: NFTIDGMGNLRITEKGLKLE GDSEFLQPLYAKEIQSRPGN ALYFKSARNVTVNILNDQTK VLTQLITGPKAVEAYGKKFE VKTVSGKLLFSADNNEVVVG AERLRVLGAEGTVFPKSIET PNVRADPFKELRLESPTRSL VMEAPKGVEINAEAGNMEAT CRTELRLESKDGEIKLDAAK IRLPRLPHGSYTPTGTRQKV FEICVCANGRLFLSQAGAGS TCQINTSVCL
Tissue Specificity Most strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
Post Translational Modifications Glycosylated. Disulfide bonds are present.
Function Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Protein Name Delta-Sarcoglycan
Delta-Sg
35 Kda Dystrophin-Associated Glycoprotein
35dag
Cellular Localisation Cell Membrane
Sarcolemma
Single-Pass Type Ii Membrane Protein
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Delta-Sarcoglycan antibody
Anti-Delta-Sg antibody
Anti-35 Kda Dystrophin-Associated Glycoprotein antibody
Anti-35dag antibody
Anti-SGCD antibody

Information sourced from Uniprot.org

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