• Immunofluorescence analysis of C6 cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA) ) Rabbit polyclonal antibody (STJ116232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA) ) Rabbit polyclonal antibody (STJ116232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using alpha Sarcoglycan (alpha Sarcoglycan (SGCA) ) Rabbit polyclonal antibody (STJ116232) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using alpha Sarcoglycan (SGCA) Rabbit polyclonal antibody (STJ116232) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SGCA antibody (60-290) (STJ116232)

SKU:
STJ116232

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SGCA (60-290) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SGCA
Gene ID: 6442
Uniprot ID: SGCA_HUMAN
Immunogen Region: 60-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human alpha Sarcoglycan (alpha Sarcoglycan (SGCA) ) (NP_000014.1).
Immunogen Sequence: ITYHAHLQGHPDLPRWLRYT QRSPHHPGFLYGSATPEDRG LQVIEVTAYNRDSFDTTRQR LVLEIGDPEGPLLPYQAEFL VRSHDAEEVLPSTPASRFLS ALGGLWEPGELQLLNVTSAL DRGGRVPLPIEGRKEGVYIK VGSASPFSTCLKMVASPDSH ARCAQGQPPLLSCYDTLAPH FRVDWCNVTLVDKSVPEPAD EVPTPGDGILEHDPFFCPPT EAPDRDFLVDA
Tissue Specificity Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain.
Function Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Protein Name Alpha-Sarcoglycan
Alpha-Sg
50 Kda Dystrophin-Associated Glycoprotein
50dag
Adhalin
Dystroglycan-2
Cellular Localisation Cell Membrane
Sarcolemma
Single-Pass Type I Membrane Protein
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Alpha-Sarcoglycan antibody
Anti-Alpha-Sg antibody
Anti-50 Kda Dystrophin-Associated Glycoprotein antibody
Anti-50dag antibody
Anti-Adhalin antibody
Anti-Dystroglycan-2 antibody
Anti-SGCA antibody
Anti-ADL antibody
Anti-DAG2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance