• Immunofluorescence analysis of paraffin-embedded mouse lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded human lung cancer using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SFTPC in paraffin-embedded mouse lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SFTPC in paraffin-embedded rat lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SFTPC in paraffin-embedded human lung using SFTPC Rabbit polyclonal antibody (STJ25509) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using SFTPC Rabbit polyclonal antibody (STJ25509) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SFTPC antibody (59-197) (STJ25509)

SKU:
STJ25509

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SFTPC (59-197) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFTPC
Gene ID: 6440
Uniprot ID: PSPC_HUMAN
Immunogen Region: 59-197
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 59-197 of human SFTPC (NP_003009.2).
Immunogen Sequence: HMSQKHTEMVLEMSIGAPEA QQRLALSEHLVTTATFSIGS TGLVVYDYQQLLIAYKPAPG TCCYIMKIAPESIPSLEALT RKVHNFQMECSLQAKPAVPT SKLGQAEGRDAGSAPSGGDP AFLGMAVSTLCGEVPLYYI
Function Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Protein Name Pulmonary Surfactant-Associated Protein C
Sp-C
Pulmonary Surfactant-Associated Proteolipid Spl(Val
Sp5
Database Links Reactome: R-HSA-5683826
Reactome: R-HSA-5688354
Reactome: R-HSA-5688849
Reactome: R-HSA-5688890
Cellular Localisation Secreted
Extracellular Space
Surface Film
Alternative Antibody Names Anti-Pulmonary Surfactant-Associated Protein C antibody
Anti-Sp-C antibody
Anti-Pulmonary Surfactant-Associated Proteolipid Spl(Val antibody
Anti-Sp5 antibody
Anti-SFTPC antibody
Anti-SFTP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance