Anti-SFN antibody (1-100) [S2MR] (STJ11102062)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal 14-3-3 sigma (1-100) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SFN |
| Gene ID | 2810 |
| Uniprot ID | 1433S_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MERASLIQKAKLAEQAERYE DMAAFMKGAVEKGEELSCEE RNLLSVAYKNVVGGQRAAWR VLSSIEQKSNEEGSEEKGPE VREYREKVETELQGVCDTVL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 sigma (P31947). |
Additional Info
| Tissue Specificity | Present mainly in tissues enriched in stratified squamous keratinizing epithelium. |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis. Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. |
| Protein Name | 14-3-3 Protein SigmaEpithelial Cell Marker Protein 1Stratifin |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-1445148Reactome: R-HSA-5625740Reactome: R-HSA-5628897Reactome: R-HSA-6804114Reactome: R-HSA-75035Reactome: R-HSA-9614399Reactome: R-HSA-9735871Reactome: R-HSA-9755779 |
| Cellular Localisation | CytoplasmNucleusSecretedMay Be Secreted By A Non-Classical Secretory Pathway |
| Alternative Antibody Names | Anti-14-3-3 Protein Sigma antibodyAnti-Epithelial Cell Marker Protein 1 antibodyAnti-Stratifin antibodyAnti-SFN antibodyAnti-HME1 antibody |
Information sourced from Uniprot.org