• Confocal immunofluorescence analysis of U2OS cells using SF3A3 Polyclonal Antibody (STJ25497) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SF3A3 antibody (STJ25497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using SF3A3 antibody (STJ25497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SF3A3 antibody (292-501) (STJ25497)

SKU:
STJ25497

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF3A3 (292-501) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3A3
Gene ID: 10946
Uniprot ID: SF3A3_HUMAN
Immunogen Region: 292-501
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3 (NP_006793.1).
Immunogen Sequence: SLESLDTSLFAKNPKSKGTK RDTERNKDIAFLEAQIYEYV EILGEQRHLTHENVQRKQAR TGEEREEEEEEQISESESED EENEIIYNPKNLPLGWDGKP IPYWLYKLHGLNINYNCEIC GNYTYRGPKAFQRHFAEWRH AHGMRCLGIPNTAHFANVTQ IEDAVSLWAKLKLQKASERW QPDTEEEYEDSSGNVVNKKT YEDLKRQGLL
Tissue Specificity Ubiquitous.
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Protein Name Splicing Factor 3a Subunit 3
Sf3a60
Spliceosome-Associated Protein 61
Sap 61
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Nucleus
Alternative Antibody Names Anti-Splicing Factor 3a Subunit 3 antibody
Anti-Sf3a60 antibody
Anti-Spliceosome-Associated Protein 61 antibody
Anti-Sap 61 antibody
Anti-SF3A3 antibody
Anti-SAP61 antibody

Information sourced from Uniprot.org

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