• Confocal immunofluorescence analysis of U2OS cells using SF3A3 Polyclonal Antibody (STJ25497) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SF3A3 Rabbit polyclonal antibody (STJ25497) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SF3A3 antibody (STJ25497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using SF3A3 antibody (STJ25497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SF3A3 antibody (292-501) (STJ25497)

SKU:
STJ25497

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3A3
Gene ID: 10946
Uniprot ID: SF3A3_HUMAN
Immunogen Region: 292-501
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 292-501 of human SF3A3 (NP_006793.1).
Immunogen Sequence: SLESLDTSLFAKNPKSKGTK RDTERNKDIAFLEAQIYEYV EILGEQRHLTHENVQRKQAR TGEEREEEEEEQISESESED EENEIIYNPKNLPLGWDGKP IPYWLYKLHGLNINYNCEIC GNYTYRGPKAFQRHFAEWRH AHGMRCLGIPNTAHFANVTQ IEDAVSLWAKLKLQKASERW QPDTEEEYEDSSGNVVNKKT YEDLKRQGLL
Tissue Specificity Ubiquitous.
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Protein Name Splicing Factor 3a Subunit 3
Sf3a60
Spliceosome-Associated Protein 61
Sap 61
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus Speckle
Nucleus
Alternative Antibody Names Anti-Splicing Factor 3a Subunit 3 antibody
Anti-Sf3a60 antibody
Anti-Spliceosome-Associated Protein 61 antibody
Anti-Sap 61 antibody
Anti-SF3A3 antibody
Anti-SAP61 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance