• Western blot analysis of various lysates using SF3A1 Rabbit polyclonal antibody (STJ11100905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SF3A1 antibody (715-785) (STJ11100905)

SKU:
STJ11100905

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF3A1 (715-785) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3A1
Gene ID: 10291
Uniprot ID: SF3A1_HUMAN
Immunogen Region: 715-785
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 715-785 of human SF3A1 (Q15459).
Immunogen Sequence: QDKTEWKLNGQVLVFTLPLT DQVSVIKVKIHEATGMPAGK QKLQYEGIFIKDSNSLAYYN MANGAVIHLAL
Tissue Specificity Ubiquitously expressed.
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Protein Name Splicing Factor 3a Subunit 1
Sf3a120
Spliceosome-Associated Protein 114
Sap 114
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Splicing Factor 3a Subunit 1 antibody
Anti-Sf3a120 antibody
Anti-Spliceosome-Associated Protein 114 antibody
Anti-Sap 114 antibody
Anti-SF3A1 antibody
Anti-SAP114 antibody

Information sourced from Uniprot.org

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