• Western blot analysis of various lysates using SF3A1 Rabbit polyclonal antibody (STJ11100905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SF3A1 antibody (715-785) (STJ11100905)

SKU:
STJ11100905

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3A1
Gene ID: 10291
Uniprot ID: SF3A1_HUMAN
Immunogen Region: 715-785
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 715-785 of human SF3A1 (Q15459).
Immunogen Sequence: QDKTEWKLNGQVLVFTLPLT DQVSVIKVKIHEATGMPAGK QKLQYEGIFIKDSNSLAYYN MANGAVIHLAL
Tissue Specificity Ubiquitously expressed.
Function Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.
Protein Name Splicing Factor 3a Subunit 1
Sf3a120
Spliceosome-Associated Protein 114
Sap 114
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Splicing Factor 3a Subunit 1 antibody
Anti-Sf3a120 antibody
Anti-Spliceosome-Associated Protein 114 antibody
Anti-Sap 114 antibody
Anti-SF3A1 antibody
Anti-SAP114 antibody

Information sourced from Uniprot.org

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