Anti-SF1 antibody (1-280) (STJ28507)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ28507
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General Information

Short DescriptionRabbit polyclonal SF1 (1-280) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolSF1
Gene ID7536
Uniprot IDSF01_HUMAN
Immunogen Region1-280
Immunogen SequenceMATGANATPLDFPSKKRKRS RWNQDTMEQKTVIPGMPTVI PPGLTREQERAYIVQLQIED LTRKLRTGDLGIPPNPEDRS PSPEPIYNSEGKRLNTREFR TRKKLEEERHNLITEMVALN PDFKPPADYKPPATRVSDKV MIPQDEYPEINFVGLLIGPR GNTLKNIEKECNAKIMIRGK GSVKEGKVGRKDGQMLPGED EPLHALVTANTMENVKKAVE QIRNILKQGIETPEDQNDL
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).

Additional Info

Tissue Specificity Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Post Translational Modifications Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Function Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Protein Name Splicing Factor 1
Mammalian Branch Point-Binding Protein
Bbp
Mbbp
Transcription Factor Zfm1
Zinc Finger Gene In Men1 Locus
Zinc Finger Protein 162
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 1 antibody
Anti-Mammalian Branch Point-Binding Protein antibody
Anti-Bbp antibody
Anti-Mbbp antibody
Anti-Transcription Factor Zfm1 antibody
Anti-Zinc Finger Gene In Men1 Locus antibody
Anti-Zinc Finger Protein 162 antibody
Anti-SF1 antibody
Anti-ZFM1 antibody
Anti-ZNF162 antibody

Information sourced from Uniprot.org

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