• Human adrenal gland stained with anti-SF-1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of cortical cells.

Anti-SF-1 antibody (200-400aa) [ZR397] (STJ180598)

SKU:
STJ180598

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Host: Rabbit
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Steroidogenic Factor-1 (200-400aa) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZR397
Conjugation: Unconjugated
Isotype: IgG
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100-200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: NR5A1
Gene ID: 2516
Uniprot ID: STF1_HUMAN
Immunogen Region: 200-400aa
Specificity: Positive Control: Adrenal cortical carcinoma
Immunogen: Recombinant fragment (around aa200-400) of human SF-1 protein
Tissue Specificity High expressed in the adrenal cortex, the ovary, the testis, and the spleen.
Post Translational Modifications Acetylation stimulates the transcriptional activity. Sumoylation reduces CDK7-mediated phosphorylation on Ser-203. Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity.
Function Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine. Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Protein Name Steroidogenic Factor 1
Sf-1
Stf-1
Hsf-1
Adrenal 4-Binding Protein
Fushi Tarazu Factor Homolog 1
Nuclear Receptor Subfamily 5 Group A Member 1
Steroid Hormone Receptor Ad4bp
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-452723
Reactome: R-HSA-9690406
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Steroidogenic Factor 1 antibody
Anti-Sf-1 antibody
Anti-Stf-1 antibody
Anti-Hsf-1 antibody
Anti-Adrenal 4-Binding Protein antibody
Anti-Fushi Tarazu Factor Homolog 1 antibody
Anti-Nuclear Receptor Subfamily 5 Group A Member 1 antibody
Anti-Steroid Hormone Receptor Ad4bp antibody
Anti-NR5A1 antibody
Anti-AD4BP antibody
Anti-FTZF1 antibody
Anti-SF1 antibody

Information sourced from Uniprot.org

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