| Host: | Mouse |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Histone-lysine N-methyltransferase SETDB1 is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 5H6A12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 IF 1:200-1:1000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SETDB1 |
| Gene ID: | 9869 |
| Uniprot ID: | SETB1_HUMAN |
| Specificity: | ESET Monoclonal Antibody detects endogenous levels of ESET protein. |
| Immunogen: | Purified recombinant fragment of human ESET expressed in E. Coli. |
| Post Translational Modifications | Degraded by the proteasome, shielded by interaction with ATF7IP. Monoubiquitinated at Lys-867 by E2 enzymes of the UBE2E family. The conjugated-Ub is protected from deubiquitination by the SET domain. Monoubiquitination at Lys-867 is required for catalytic activity, H3K9 methylation and endogenous retrovirus silencing. |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. |
| Protein Name | Histone-Lysine N-Methyltransferase Setdb1 Erg-Associated Protein With Set Domain Eset Histone H3-K9 Methyltransferase 4 H3-K9-Hmtase 4 Lysine N-Methyltransferase 1e Set Domain Bifurcated 1 |
| Database Links | Reactome: R-HSA-3214841 Reactome: R-HSA-9843940 Reactome: R-HSA-9843970 |
| Cellular Localisation | Nucleus Cytoplasm Chromosome Associated With Non-Pericentromeric Regions Of Chromatin Excluded From Nucleoli And Islands Of Condensed Chromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setdb1 antibody Anti-Erg-Associated Protein With Set Domain antibody Anti-Eset antibody Anti-Histone H3-K9 Methyltransferase 4 antibody Anti-H3-K9-Hmtase 4 antibody Anti-Lysine N-Methyltransferase 1e antibody Anti-Set Domain Bifurcated 1 antibody Anti-SETDB1 antibody Anti-ESET antibody Anti-KIAA0067 antibody Anti-KMT1E antibody |
Information sourced from Uniprot.org