• Immunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 Rabbit polyclonal antibody (STJ11103839) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using HSP47/SERPINH1 antibody (STJ11103839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SERPINH1 antibody (139-418) (STJ11103839)

SKU:
STJ11103839

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINH1
Gene ID: 871
Uniprot ID: SERPH_HUMAN
Immunogen Region: 139-418
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 139-418 of human HSP47/HSP47/SERPINH1 (NP_001226.2).
Immunogen Sequence: SVSFADDFVRSSKQHYNCEH SKINFRDKRSALQSINEWAA QTTDGKLPEVTKDVERTDGA LLVNAMFFKPHWDEKFHHKM VDNRGFMVTRSYTVGVMMMH RTGLYNYYDDEKEKLQIVEM PLAHKLSSLIILMPHHVEPL ERLEKLLTKEQLKIWMGKMQ KKAVAISLPKGVVEVTHDLQ KHLAGLGLTEAIDKNKADLS RMSGKKDLYLASVFHATAFE LDTDGNPFDQDIYGREELR
Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Protein Name Serpin H1
47 Kda Heat Shock Protein
Arsenic-Transactivated Protein 3
Astp3
Cell Proliferation-Inducing Gene 14 Protein
Collagen-Binding Protein
Colligin
Rheumatoid Arthritis-Related Antigen Ra-A47
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Serpin H1 antibody
Anti-47 Kda Heat Shock Protein antibody
Anti-Arsenic-Transactivated Protein 3 antibody
Anti-Astp3 antibody
Anti-Cell Proliferation-Inducing Gene 14 Protein antibody
Anti-Collagen-Binding Protein antibody
Anti-Colligin antibody
Anti-Rheumatoid Arthritis-Related Antigen Ra-A47 antibody
Anti-SERPINH1 antibody
Anti-CBP1 antibody
Anti-CBP2 antibody
Anti-HSP47 antibody
Anti-SERPINH2 antibody
Anti-PIG14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance