![Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100497/387047/STJ25485_1__70610.1713156238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100497/387047/STJ25485_1__70610.1713156238.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SERPINB5 |
| Gene ID: | 5268 |
| Uniprot ID: | SPB5_HUMAN |
| Immunogen Region: | 21-189 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-189 of human SERPINB5 (NP_002630.2). |
| Immunogen Sequence: | EKEPLGNVLFSPICLSTSLS LAQVGAKGDTANEIGQVLHF ENVKDVPFGFQTVTSDVNKL SSFYSLKLIKRLYVDKSLNL STEFISSTKRPYAKELETVD FKDKLEETKGQINNSIKDLT DGHFENILADNSVNDQTKIL VVNAAYFVGKWMKKFSESET KECPFRVNK |
| Tissue Specificity | Normal mammary epithelial cells. |
| Function | Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Protein Name | Serpin B5MaspinPeptidase Inhibitor 5Pi-5 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Names | Anti-Serpin B5 antibodyAnti-Maspin antibodyAnti-Peptidase Inhibitor 5 antibodyAnti-Pi-5 antibodyAnti-SERPINB5 antibodyAnti-PI5 antibody |
Information sourced from Uniprot.org