• Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa (KO) cells, using [KO Validated] SERPINB5 Rabbit polyclonal antibody (STJ25485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SERPINB5 antibody (21-189) (STJ25485)

SKU:
STJ25485

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINB5 (21-189) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINB5
Gene ID: 5268
Uniprot ID: SPB5_HUMAN
Immunogen Region: 21-189
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-189 of human SERPINB5 (NP_002630.2).
Immunogen Sequence: EKEPLGNVLFSPICLSTSLS LAQVGAKGDTANEIGQVLHF ENVKDVPFGFQTVTSDVNKL SSFYSLKLIKRLYVDKSLNL STEFISSTKRPYAKELETVD FKDKLEETKGQINNSIKDLT DGHFENILADNSVNDQTKIL VVNAAYFVGKWMKKFSESET KECPFRVNK
Tissue Specificity Normal mammary epithelial cells.
Function Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Protein Name Serpin B5
Maspin
Peptidase Inhibitor 5
Pi-5
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Serpin B5 antibody
Anti-Maspin antibody
Anti-Peptidase Inhibitor 5 antibody
Anti-Pi-5 antibody
Anti-SERPINB5 antibody
Anti-PI5 antibody

Information sourced from Uniprot.org

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