• Western blot analysis of extracts of Human plasma, using Kallistatin (SERPINA4) rabbit polyclonal antibody antibody (STJ29459) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using Kallistatin (SERPINA4) rabbit polyclonal antibody antibody (STJ29459) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of MCF7 cells using Kallistatin (SERPINA4) antibody (STJ29459). Blue: DAPI for nuclear staining.

Anti-SERPINA4 antibody (178-427) (STJ29459)

SKU:
STJ29459

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINA4 (178-427) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINA4
Gene ID: 5267
Uniprot ID: KAIN_HUMAN
Immunogen Region: 178-427
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human Kallistatin (SERPINA4) (NP_006206.2).
Immunogen Sequence: TIQLINDHVKKETRGKIVDL VSELKKDVLMVLVNYIYFKA LWEKPFISSRTTPKDFYVDE NTTVRVPMMLQDQEHHWYLH DRYLPCSVLRMDYKGDATVF FILPNQGKMREIEEVLTPEM LMRWNNLLRKRNFYKKLELH LPKFSISGSYVLDQILPRLG FTDLFSKWADLSGITKQQKL EASKSFHKATLDVDEAGTEA AAATSFAIKFFSAQTNRHIL RFNRPFLVVIFSTSTQSVL
Tissue Specificity Expressed by the liver and secreted in plasma.
Post Translational Modifications The N-terminus is blocked.
Function Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
Protein Name Kallistatin
Kallikrein Inhibitor
Peptidase Inhibitor 4
Pi-4
Serpin A4
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Alternative Antibody Names Anti-Kallistatin antibody
Anti-Kallikrein Inhibitor antibody
Anti-Peptidase Inhibitor 4 antibody
Anti-Pi-4 antibody
Anti-Serpin A4 antibody
Anti-SERPINA4 antibody
Anti-KST antibody
Anti-PI4 antibody

Information sourced from Uniprot.org

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