• Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CD62L/L-Selectin antibody (STJ25469) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using CD62L/L-Selectin antibody (STJ25469) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using CD62L/L-Selectin antibody (STJ25469) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat thymus, using CD62L/L-Selectin antibody (STJ25469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using CD62L/L-Selectin antibody (STJ25469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SELL antibody (56-69) (STJ25469)

SKU:
STJ25469

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SELL (56-69) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SELL
Gene ID: 6402
Uniprot ID: LYAM1_HUMAN
Immunogen Region: 56-69
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 56-69 of human CD62L/L-Selectin (NP_000646.2).
Immunogen Sequence: YSEKPMNWQRARRF
Tissue Specificity Expressed in B-cell lines and T-lymphocytes.
Post Translational Modifications N-glycosylated.
Function Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Protein Name L-Selectin
Cd62 Antigen-Like Family Member L
Leukocyte Adhesion Molecule 1
Lam-1
Leukocyte Surface Antigen Leu-8
Leukocyte-Endothelial Cell Adhesion Molecule 1
Lecam1
Lymph Node Homing Receptor
Tq1
Gp90-Mel
Cd Antigen Cd62l
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-202733
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-L-Selectin antibody
Anti-Cd62 Antigen-Like Family Member L antibody
Anti-Leukocyte Adhesion Molecule 1 antibody
Anti-Lam-1 antibody
Anti-Leukocyte Surface Antigen Leu-8 antibody
Anti-Leukocyte-Endothelial Cell Adhesion Molecule 1 antibody
Anti-Lecam1 antibody
Anti-Lymph Node Homing Receptor antibody
Anti-Tq1 antibody
Anti-Gp90-Mel antibody
Anti-Cd Antigen Cd62l antibody
Anti-SELL antibody
Anti-LNHR antibody
Anti-LYAM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance