• Immunofluorescence analysis of U2OS cells using SECISBP2 Rabbit polyclonal antibody (STJ28819) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SECISBP2 Rabbit polyclonal antibody (STJ28819) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SECISBP2 Rabbit polyclonal antibody (STJ28819) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SECISBP2 antibody (STJ28819) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using SECISBP2 antibody (STJ28819) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SECISBP2 antibody (STJ28819) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SECISBP2 antibody (585-854) (STJ28819)

SKU:
STJ28819

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SECISBP2 (585-854) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SECISBP2
Gene ID: 79048
Uniprot ID: SEBP2_HUMAN
Immunogen Region: 585-854
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 585-854 of human SECISBP2 (NP_076982.3).
Immunogen Sequence: DELISTPSVEDKSEEPPGTE LQRDTEASHLAPNHTTFPKI HSRRFRDYCSQMLSKEVDAC VTDLLKELVRFQDRMYQKDP VKAKTKRRLVLGLREVLKHL KLKKLKCVIISPNCEKIQSK GGLDDTLHTIIDYACEQNIP FVFALNRKALGRSLNKAVPV SVVGIFSYDGAQDQFHKMVE LTVAARQAYKTMLENVQQEL VGEPRPQAPPSLPTQGPSCP AEDGPPALKEKEEPHYIEI
Tissue Specificity Expressed at high levels in testis.
Function Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB.
Protein Name Selenocysteine Insertion Sequence-Binding Protein 2
Secis-Binding Protein 2
Database Links Reactome: R-HSA-2408557
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Mitochondrion
Alternative Antibody Names Anti-Selenocysteine Insertion Sequence-Binding Protein 2 antibody
Anti-Secis-Binding Protein 2 antibody
Anti-SECISBP2 antibody
Anti-SBP2 antibody

Information sourced from Uniprot.org

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