• Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and SEC61B knockout (KO) 293T cells, using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SEC61B antibody (1-96) (STJ118233)

SKU:
STJ118233

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SEC61B (1-96) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC61B
Gene ID: 10952
Uniprot ID: SC61B_HUMAN
Immunogen Region: 1-96
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
Immunogen Sequence: MPGPTPSGTNVGSSGRSPSK AVAARAAGSTVRQRKNASCG TRSAGRTTSAGTGGMWRFYT EDSPGLKVGPVPVLVMSLLF IASVFMLHIWGKYTRS
Function Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER.
Protein Name Protein Transport Protein Sec61 Subunit Beta
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1799339
Reactome: R-HSA-9609523
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Protein Transport Protein Sec61 Subunit Beta antibody
Anti-SEC61B antibody

Information sourced from Uniprot.org

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