• Immunofluorescence analysis of U-2 OS cells using Sec23B Rabbit polyclonal antibody (STJ117325) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using Sec23B Rabbit polyclonal antibody (STJ117325) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Sec23B Rabbit polyclonal antibody (STJ117325) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Sec23B Rabbit polyclonal antibody (STJ117325) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SEC23B antibody (150-250) (STJ117325)

SKU:
STJ117325

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SEC23B
Gene ID: 10483
Uniprot ID: SC23B_HUMAN
Immunogen Region: 150-250
Specificity: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Sec23B (NP_006354.2).
Immunogen Sequence: SLQMSLSLLPPDALVGLITF GRMVQVHELSCEGISKSYVF RGTKDLTAKQIQDMLGLTKP AMPMQQARPAQPQEHPFASS RFLQPVHKIDMNLTDLLGEL Q
Tissue Specificity Ubiquitously expressed.
Function Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.
Protein Name Protein Transport Protein Sec23b
Hsec23b
Sec23-Related Protein B
Cellular Localisation Cytoplasmic Vesicle
Copii-Coated Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Endoplasmic Reticulum Membrane
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Protein Transport Protein Sec23b antibody
Anti-Hsec23b antibody
Anti-Sec23-Related Protein B antibody
Anti-SEC23B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance