• Immunohistochemistry analysis of SDF4 in paraffin-embedded Human liver using SDF4 Rabbit polyclonal antibody (STJ117638) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SDF4 in paraffin-embedded Human colon using SDF4 Rabbit polyclonal antibody (STJ117638) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SDF4 Rabbit polyclonal antibody (STJ117638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SDF4 antibody (37-200) (STJ117638)

SKU:
STJ117638

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SDF4 (37-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDF4
Gene ID: 51150
Uniprot ID: CAB45_HUMAN
Immunogen Region: 37-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SDF4 (NP_057260.2).
Immunogen Sequence: RPANHSSTRERVANREENEI LPPDHLNGVKLEMDGHLNRG FHQEVFLGKDLGGFDEDAEP RRSRRKLMVIFSKVDVNTDR KISAKEMQRWIMEKTAEHFQ EAMEESKTHFRAVDPDGDGH VSWDEYKVKFLASKGHSEKE VADAIRLNEELKVDEETQEV LENL
Tissue Specificity Ubiquitous. Isoform 5 is expressed in pancreas.
Function May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini.
Protein Name 45 Kda Calcium-Binding Protein
Cab45
Stromal Cell-Derived Factor 4
Sdf-4
Cellular Localisation Isoform 1: Golgi Apparatus Lumen
Isoform 5: Cytoplasm
Cell Membrane
Cell Projection
Bleb
Isoform 5 Colocalizes With Stx3 And Stxbp1 Isoform 2 At The Plasma Membrane And Cell Surface Blebs
Alternative Antibody Names Anti-45 Kda Calcium-Binding Protein antibody
Anti-Cab45 antibody
Anti-Stromal Cell-Derived Factor 4 antibody
Anti-Sdf-4 antibody
Anti-SDF4 antibody
Anti-CAB45 antibody
Anti-PSEC0034 antibody

Information sourced from Uniprot.org

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