| Host: |
Goat |
| Applications: |
Pep-ELISA/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-SCN5A (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IHC-5-10µg/mlIF-Strong expression of the protein seen in the plasma membrane and cytoplasm/endoplasmic reticulum of U2OS cells. 10µg/mlELISA-antibody detection limit dilution 1:1000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
SCN5A |
| Gene ID: |
6331 |
| Uniprot ID: |
SCN5A_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_932173.1; NP_000326.2; NP_001092874.1; NP_001092875.1; NP_001153632.1; NP_001153633.1 |
| Specificity: |
This antibody is expected to recognise all reported isoforms (NP_932173.1; NP_000326.2; NP_001092874.1; NP_001092875.1). |
| Immunogen Sequence: |
ETDDQSPEKIN |
| Post Translational Modifications | Ubiquitinated by NEDD4L.which promotes its endocytosis. Does not seem to be ubiquitinated by NEDD4 or WWP2. Phosphorylation at Ser-1503 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents (Probable). Regulated through phosphorylation by CaMK2D. Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 868) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4. |
| Function | This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels. |
| Protein Name | Sodium Channel Protein Type 5 Subunit AlphaSodium Channel Protein Cardiac Muscle Subunit AlphaSodium Channel Protein Type V Subunit AlphaVoltage-Gated Sodium Channel Subunit Alpha Nav1.5Hh1 |
| Database Links | Reactome: R-HSA-445095Reactome: R-HSA-5576892 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmPerinuclear RegionSarcolemmaT-TubuleCell JunctionRangrf Promotes Trafficking To The Cell MembraneColocalizes With Pkp2 At Intercalated Disks In The Heart |
| Alternative Antibody Names | Anti-Sodium Channel Protein Type 5 Subunit Alpha antibodyAnti-Sodium Channel Protein Cardiac Muscle Subunit Alpha antibodyAnti-Sodium Channel Protein Type V Subunit Alpha antibodyAnti-Voltage-Gated Sodium Channel Subunit Alpha Nav1.5 antibodyAnti-Hh1 antibodyAnti-SCN5A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
