| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SAV1 (199-383) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SAV1 |
| Gene ID: |
60485 |
| Uniprot ID: |
SAV1_HUMAN |
| Immunogen Region: |
199-383 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 199-383 of human SAV1 (NP_068590.1). |
| Immunogen Sequence: |
LPLPPGWSVDWTMRGRKYYI DHNTNTTHWSHPLEREGLPP GWERVESSEFGTYYVDHTNK KAQYRHPCAPSVPRYDQPPP VTYQPQQTERNQSLLVPANP YHTAEIPDWLQVYARAPVKY DHILKWELFQLADLDTYQGM LKLLFMKELEQIVKMYEAYR QALLTELENRKQRQQWYAQQ HGKNF |
| Tissue Specificity | Ubiquitously expressed in adult tissues with highest expression in the pancreas, aorta and interventricular septum and lowest expression in skeletal muscle. Expression was higher in fetal than in the adult heart. Expressed in various cell lines. |
| Post Translational Modifications | Phosphorylated by STK3/MST2 and STK4/MST1. Phosphorylation is not required for SAV1 stability and may increase the number of protein binding sites on the scaffold molecule. |
| Function | Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. |
| Protein Name | Protein Salvador Homolog 145 Kda Ww Domain ProteinHww45 |
| Database Links | Reactome: R-HSA-2028269 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Protein Salvador Homolog 1 antibodyAnti-45 Kda Ww Domain Protein antibodyAnti-Hww45 antibodyAnti-SAV1 antibodyAnti-WW45 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
