| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SART1 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SART1 |
| Gene ID: | 9092 |
| Uniprot ID: | SNUT1_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_005137.1 |
| Specificity: | This antibody is expected to recognise both the human SART1 (800) and SART1 (259) proteins. |
| Immunogen Sequence: | GSSKKHRGEKEAA |
| Post Translational Modifications | Sumoylated with SUMO2. |
| Function | Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. |
| Protein Name | U4/U6.U5 Tri-Snrnp-Associated Protein 1Snu66 HomologHsnu66Squamous Cell Carcinoma Antigen Recognized By T-Cells 1Sart-1Hsart-1U4/U6.U5 Tri-Snrnp-Associated 110 Kda ProteinAllergen Hom S 1 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusFound In The Nucleus Of Mitogen-Activated Peripheral Blood Mononuclear Cells (Pbmcs)Tumor CellsOr Normal Cell LinesBut Not In Normal Tissues Except Testis And Fetal Liver Or In Unstimulated PbmcsSuggesting Preferential Expression In Proliferating Cells |
| Alternative Antibody Names | Anti-U4/U6.U5 Tri-Snrnp-Associated Protein 1 antibodyAnti-Snu66 Homolog antibodyAnti-Hsnu66 antibodyAnti-Squamous Cell Carcinoma Antigen Recognized By T-Cells 1 antibodyAnti-Sart-1 antibodyAnti-Hsart-1 antibodyAnti-U4/U6.U5 Tri-Snrnp-Associated 110 Kda Protein antibodyAnti-Allergen Hom S 1 antibodyAnti-SART1 antibody |
Information sourced from Uniprot.org