• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike S2 antibody (STJ11103256). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 antibody (STJ11103256) at a dilution of 1:3000.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike S2 Protein, using SARS-CoV-2 Spike S2 Rabbit polyclonal antibody (STJ11103256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SARS-CoV-2-Spike S2 antibody (1174-1273) (STJ11103256)

SKU:
STJ11103256

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Host: Rabbit
Applications: WB/IP
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-Spike S2 (1174-1273) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1174-1273
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of SARS-CoV-2 Spike S2 (YP_009724390.1).
Immunogen Sequence: ASVVNIQKEIDRLNEVAKNL NESLIDLQELGKYEQYIKWP WYIWLGFIAGLIAIVMVTIM LCCMTSCCSCLKGCCSCGSC CKFDEDDSEPVLKGVKLHYT
Background Eukaryotic translation initiation factor-3 (eIF3) , the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex.

Information sourced from Uniprot.org

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