• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike antibody (STJ11101794). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (STJ11101794) at a dilution of 1:1000.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike protein and untreated 293T cells use SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV-2 S1+S2 ECD (S-ECD) Protein (RP01283) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) with a linear range of 0. 78-50ng/mL.
  • Western blot analysis of extracts of 293T and transfected 293T-S ECD (His-tag) , using SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at 1:1000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of 293T and transfected 293T-S1 (His-tag) , using SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2 Spike antibody (600-700) (STJ11101794)

SKU:
STJ11101794

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Spike (600-700) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 600-700
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of coronavirus Spike (YP_009724390.1).
Immunogen Sequence: PGTNTSNQVAVLYQDVNCTE VPVAIHADQLTPTWRVYSTG SNVFQTRAGCLIGAEHVNNS YECDIPIGAGICASYQTQTN SPRRARSVASQSIIAYTMSL G
Background This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it s believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.

Information sourced from Uniprot.org

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