• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike antibody (STJ11101794). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (STJ11101794) at a dilution of 1:1000.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike protein and untreated 293T cells use SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV-2 S1+S2 ECD (S-ECD) Protein (RP01283) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) with a linear range of 0. 78-50ng/mL.
  • Western blot analysis of extracts of 293T and transfected 293T-S ECD (His-tag) , using SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at 1:1000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of 293T and transfected 293T-S1 (His-tag) , using SARS-CoV-2 Spike Rabbit polyclonal antibody (STJ11101794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SARS-CoV-2 Spike antibody (600-700) (STJ11101794)

SKU:
STJ11101794

£112.50 - £524.50
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Host: Rabbit
Applications: WB/DB/IF/ICC/IP/ELISA
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
DB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specifi
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 600-700
Specificity: A synthetic peptide corresponding to a sequence within amino acids 600-700 of coronavirus Spike (YP_009724390.1).
Immunogen Sequence: PGTNTSNQVAVLYQDVNCTE VPVAIHADQLTPTWRVYSTG SNVFQTRAGCLIGAEHVNNS YECDIPIGAGICASYQTQTN SPRRARSVASQSIIAYTMSL G
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E) , spike or surface glycoprotein (S) , membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Information sourced from Uniprot.org

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