• Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 N Protein Mouse monoclonal antibody (STJ11103176) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immobilized Recombinant 2019-nCoV Nucleocapsid Protein (RP01264LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Nucleoprotein Rabbit monoclonal antibody (STJ11103176) with a linear range of 3. 12-200ng/mL.
  • Western blot analysis of extracts of normal 293T cells 293T transfected with N Protein, using SARS-CoV-2 N Protein Mouse monoclonal antibody (STJ11103176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SARS-CoV-2-N Protein antibody (1-420 aa) [S6MM] (STJ11103176)

SKU:
STJ11103176

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Host: Mouse
Applications: WB/IF/ICC/ELISA
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S6MM
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IF/ICC:1:50-1:200
ELISA:1:30000-1:120000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-420 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Immunogen Sequence: MSDNGPQSNQRSAPRITFGG PTDSTDNNQNGGRNGARPKQ RRPQGLPNNTASWFTALTQH GKEELRFPRGQGVPINTNSG PDDQIGYYRRATRRVRGGDG KMKELSPRWYFYYLGTGPEA SLPYGANKEGIVWVATEGAL NTPKDHIGTRNPNNNAATVL QLPQGTTLPKGFYAEGSRGG SQASSRSSSRSRGNSRNSTP GSSRGNSPARMASGGGETAL ALLLLDRLNQLESKVSGKG
Background Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host SMAD3.

Information sourced from Uniprot.org

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