Anti-SARM1 antibody (625-724) (STJ11103379)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SARM1 (625-724) antibody for use in WB, IP and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SARM1 |
| Gene ID | 23098 |
| Uniprot ID | SARM1_HUMAN |
| Immunogen Region | 625-724 |
| Immunogen Sequence | ALDKCMQDHDCKDWVHKEIV TALSCGKNIVPIIDGFEWPE PQVLPEDMQAVLTFNGIKWS HEYQEATIEKIIRFLQGRSS RDSSAGSDTSLEGAAPMGPT |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 625-724 of human SARM1 (NP_055892.2). |
Additional Info
| Tissue Specificity | Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta. |
| Post Translational Modifications | Phosphorylation at Ser-548 by JNK kinases (MAPK8, MAPK9 and /or MAPK10) enhance the NAD(+) hydrolase (NADase) activity. Phosphorylation at Ser-548 and subsequent activation takes place in response to oxidative stress conditions and inhibits mitochondrial respiration. |
| Function | NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide.NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38. |
| Protein Name | Nad(+ Hydrolase Sarm1Nadase Sarm1Hsarm1Nadp(+ Hydrolase Sarm1Sterile Alpha And Armadillo Repeat ProteinSterile Alpha And Tir Motif-Containing Protein 1Sterile Alpha Motif Domain-Containing Protein 2Myd88-5Sam Domain-Containing Protein 2Tir-1 HomologHstir |
| Database Links | Reactome: R-HSA-166166 Q6SZW1-1Reactome: R-HSA-168164 Q6SZW1-1Reactome: R-HSA-936964 Q6SZW1-1Reactome: R-HSA-937041 Q6SZW1-1Reactome: R-HSA-937072 Q6SZW1-1 |
| Cellular Localisation | CytoplasmCell ProjectionAxonDendriteSynapseMitochondrionAssociated With Microtubules |
| Alternative Antibody Names | Anti-Nad(+ Hydrolase Sarm1 antibodyAnti-Nadase Sarm1 antibodyAnti-Hsarm1 antibodyAnti-Nadp(+ Hydrolase Sarm1 antibodyAnti-Sterile Alpha And Armadillo Repeat Protein antibodyAnti-Sterile Alpha And Tir Motif-Containing Protein 1 antibodyAnti-Sterile Alpha Motif Domain-Containing Protein 2 antibodyAnti-Myd88-5 antibodyAnti-Sam Domain-Containing Protein 2 antibodyAnti-Tir-1 Homolog antibodyAnti-Hstir antibodyAnti-SARM1 antibodyAnti-KIAA0524 antibodyAnti-SAMD2 antibodyAnti-SARM antibody |
Information sourced from Uniprot.org