• Western blot analysis of extracts of various cell lines, using SARDH Antibody (STJ113655) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using SARDH Rabbit polyclonal antibody (STJ113655) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using SARDH Polyclonal Antibody (STJ113655) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using SARDH Rabbit polyclonal antibody (STJ113655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-SARDH antibody (300-560) (STJ113655)

SKU:
STJ113655

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARDH (300-560) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SARDH
Gene ID: 1757
Uniprot ID: SARDH_HUMAN
Immunogen Region: 300-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2).
Immunogen Sequence: RIEGIQNMPNVRDHDASVYL RLQGDALSVGGYEANPIFWE EVSDKFAFGLFDLDWEVFTQ HIEGAINRVPVLEKTGIKST VCGPESFTPDHKPLMGEAPE LRGFFLGCGFNSAGMMLGGG CGQELAHWIIHGRPEKDMHG YDIRRFHHSLTDHPRWIRER SHESYAKNYSVVFPHDEPLA GRNMRRDPLHEELLGQGCVF QERHGWERPGWFHPRGPAPV LEYDYYGAYGSRAHEDYAY
Tissue Specificity Expressed in pancreas, liver and kidney.
Function Catalyzes the last step of the oxidative degradation of choline to glycine. Converts sarcosine into glycine.
Protein Name Sarcosine Dehydrogenase - Mitochondrial
Sardh
Bpr-2
Database Links Reactome: R-HSA-6798163
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Sarcosine Dehydrogenase - Mitochondrial antibody
Anti-Sardh antibody
Anti-Bpr-2 antibody
Anti-SARDH antibody
Anti-DMGDHL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance