| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-SAMHD1 (527-626) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S0MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SAMHD1 |
| Gene ID: |
25939 |
| Uniprot ID: |
SAMH1_HUMAN |
| Immunogen Region: |
527-626 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 527-626 of human SAMHD1 (NP_056289.2). |
| Immunogen Sequence: |
NRAIRITKNQVSQLLPEKFA EQLIRVYCKKVDRKSLYAAR QYFVQWCADRNFTKPQDGDV IAPLITPQKKEWNDSTSVQN PTRLREASKSRVQLFKDDPM |
| Tissue Specificity | Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus. |
| Post Translational Modifications | Phosphorylation at Thr-592 by CDK1 acts as a switch to control deoxynucleoside triphosphate (dNTPase)-dependent and -independent functions. Phosphorylation at Thr-592 takes place in cycling cells: it reduces the stability of the homotetramer, impairing the dNTPase activity and subsequent ability to restrict infection by viruses. It also inhibits ability to suppress LINE-1 retrotransposon activity. In contrast, phosphorylation at Thr-592 promotes DNA end resection at stalled replication forks in response to DNA damage. (Microbial infection) Phosphorylation at Thr-592 by Epstein-Barr virus kinase BGLF4 and human cytomegalovirus/HCMV UL97 leads to a reduced level of dCTPase and dTTPase activity and the loss of viral restriction. (Microbial infection) Ubiquitinated following interaction with HIV-2 viral protein Vpx.Vpx promotes interaction and with a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase, leading to proteasomal degradation. |
| Function | Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks. Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. Not able to restrict infection by HIV-2 virus.because restriction activity is counteracted by HIV-2 viral protein Vpx. In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools. Phosphorylation at Thr-592 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks. Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication. Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation. Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity. Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation. |
| Protein Name | Deoxynucleoside Triphosphate Triphosphohydrolase Samhd1DntpaseDendritic Cell-Derived Ifng-Induced ProteinDcipMonocyte Protein 5Mop-5Sam Domain And Hd Domain-Containing Protein 1Hsamhd1 |
| Database Links | Reactome: R-HSA-8956319Reactome: R-HSA-909733 |
| Cellular Localisation | NucleusChromosomeLocalizes To Sites Of Dna Double-Strand Breaks In Response To Dna Damage |
| Alternative Antibody Names | Anti-Deoxynucleoside Triphosphate Triphosphohydrolase Samhd1 antibodyAnti-Dntpase antibodyAnti-Dendritic Cell-Derived Ifng-Induced Protein antibodyAnti-Dcip antibodyAnti-Monocyte Protein 5 antibodyAnti-Mop-5 antibodyAnti-Sam Domain And Hd Domain-Containing Protein 1 antibodyAnti-Hsamhd1 antibodyAnti-SAMHD1 antibodyAnti-MOP5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
