• Western blot analysis of extracts of various cell lines, using SALL4 Antibody (STJ29204) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.
  • Immunofluorescence analysis of mouse testis cells using SALL4 rabbit polyclonal antibody (STJ29204) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SALL4 antibody (1-220) (STJ29204)

SKU:
STJ29204

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SALL4 (1-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SALL4
Gene ID: 57167
Uniprot ID: SALL4_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Sall4 (NP_065169.1).
Immunogen Sequence: MSRRKQAKPQHINSEEDQGE QQPQQQTPEFADAAPAAPAA GELGAPVNHPGNDEVASEDE ATVKRLRREETHVCEKCCAE FFSISEFLEHKKNCTKNPPV LIMNDSEGPVPSEDFSGAVL SHQPTSPGSKDCHRENGGSS EDMKEKPDAESVVYLKTETA LPPTPQDISYLAKGKVANTN VTLQALRGTKVAVNQRSADA LPAPVPGANSIPWVLEQILC
Tissue Specificity Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML).
Post Translational Modifications Isoform SALL4B exists primarily as a ubiquitinated form. Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4.
Function Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells.
Protein Name Sal-Like Protein 4
Zinc Finger Protein 797
Zinc Finger Protein Sall4
Database Links Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-8943724
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Sal-Like Protein 4 antibody
Anti-Zinc Finger Protein 797 antibody
Anti-Zinc Finger Protein Sall4 antibody
Anti-SALL4 antibody
Anti-ZNF797 antibody

Information sourced from Uniprot.org

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