Western blot analysis of extracts of various cell lines, using S1PR3 Rabbit monoclonal antibody (STJ11101433) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-S1PR3 antibody (1-100) [S3MR] (STJ11101433)

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STJ11101433
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S1PR3
Gene ID: 1903
Uniprot ID: S1PR3_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human S1PR3 (Q99500).
Immunogen Sequence: MATALPPRLQPVRGNETLRE HYQYVGKLAGRLKEASEGST LTTVLFLVICSFIVLENLMV LIAIWKNNKFHNRMYFFIGN LALCDLLAGIAYKVNILMSG
Tissue Specificity Expressed in all tissues, but most abundantly in heart, placenta, kidney, and liver.
Function Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Protein Name Sphingosine 1-Phosphate Receptor 3
S1p Receptor 3
S1p3
Endothelial Differentiation G-Protein Coupled Receptor 3
Sphingosine 1-Phosphate Receptor Edg-3
S1p Receptor Edg-3
Database Links Reactome: R-HSA-418594
Reactome: R-HSA-419408
Reactome: R-HSA-9009391
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Sphingosine 1-Phosphate Receptor 3 antibody
Anti-S1p Receptor 3 antibody
Anti-S1p3 antibody
Anti-Endothelial Differentiation G-Protein Coupled Receptor 3 antibody
Anti-Sphingosine 1-Phosphate Receptor Edg-3 antibody
Anti-S1p Receptor Edg-3 antibody
Anti-S1PR3 antibody
Anti-C9orf108 antibody
Anti-C9orf47 antibody
Anti-EDG3 antibody

Information sourced from Uniprot.org

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