Anti-S1PR1 antibody (1-100) [S2MR] (STJ11101982)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal EDG1 (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S2MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | S1PR1 |
| Gene ID | 1901 |
| Uniprot ID | S1PR1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MGPTSVPLVKAHRSSVSDYV NYDIIVRHYNYTGKLNISAD KENSIKLTSVVFILICCFII LENIFVLLTIWKTKKFHRPM YYFIGNLALSDLLAGVAYTA |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EDG1/HEXIM1 (P21453). |
Additional Info
| Tissue Specificity | Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. |
| Post Translational Modifications | S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. Palmitoylated by ZDHHC5. Palmitoylation is required for targeting to plasma membrane, enabling G(i) coupling. |
| Function | G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that seems to be coupled to the G(i) subclass of heteromeric G proteins. Signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. Plays an important role in cell migration, probably via its role in the reorganization of the actin cytoskeleton and the formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. Required for normal chemotaxis toward sphingosine 1-phosphate. Required for normal embryonic heart development and normal cardiac morphogenesis. Plays an important role in the regulation of sprouting angiogenesis and vascular maturation. Inhibits sprouting angiogenesis to prevent excessive sprouting during blood vessel development. Required for normal egress of mature T-cells from the thymus into the blood stream and into peripheral lymphoid organs. Plays a role in the migration of osteoclast precursor cells, the regulation of bone mineralization and bone homeostasis. Plays a role in responses to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine by pulmonary endothelial cells and in the protection against ventilator-induced lung injury. |
| Protein Name | Sphingosine 1-Phosphate Receptor 1S1p Receptor 1S1p1Endothelial Differentiation G-Protein Coupled Receptor 1Sphingosine 1-Phosphate Receptor Edg-1S1p Receptor Edg-1Cd Antigen Cd363 |
| Database Links | Reactome: R-HSA-419408Reactome: R-HSA-6785807Reactome: R-HSA-9679191 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndosomeMembrane RaftRecruited To Caveolin-Enriched Plasma Membrane Microdomains In Response To Oxidized 1-Palmitoyl-2-Arachidonoyl-Sn-Glycero-3-PhosphocholineLigand Binding Leads To Receptor Internalization |
| Alternative Antibody Names | Anti-Sphingosine 1-Phosphate Receptor 1 antibodyAnti-S1p Receptor 1 antibodyAnti-S1p1 antibodyAnti-Endothelial Differentiation G-Protein Coupled Receptor 1 antibodyAnti-Sphingosine 1-Phosphate Receptor Edg-1 antibodyAnti-S1p Receptor Edg-1 antibodyAnti-Cd Antigen Cd363 antibodyAnti-S1PR1 antibodyAnti-CHEDG1 antibodyAnti-EDG1 antibody |
Information sourced from Uniprot.org