Anti-S100A4/CAPL antibody (C-Term) (STJ71033)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ71033-100
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General Information

Short DescriptionGoat polyclonal antibody anti-S100A4/CAPL (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
ApplicationsPep-ELISA/WB/IHC
HostGoat
ReactivityHuman/Mouse/Rat/Dog
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 13kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7kDa according to NP_002952.1). Recommended concentration: 0.05-0.2ยตg/ml. Primary i
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolS100A4
Gene ID6275
Uniprot IDS10A4_HUMAN
Accession NumberNP_002952.1; NP_062427.1
Immunogen RegionC-Term
Immunogen SequenceCNEFFEGFPDKQP
SpecificityBoth variants (NP_002952.1; NP_062427.1) represent identical protein.

Additional Info

Function Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy. Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9. Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis. Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels. Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes. In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors.
Protein Name Protein S100-A4
Calvasculin
Metastasin
Placental Calcium-Binding Protein
Protein Mts1
S100 Calcium-Binding Protein A4
Cellular Localisation Secreted
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Protein S100-A4 antibody
Anti-Calvasculin antibody
Anti-Metastasin antibody
Anti-Placental Calcium-Binding Protein antibody
Anti-Protein Mts1 antibody
Anti-S100 Calcium-Binding Protein A4 antibody
Anti-S100A4 antibody
Anti-CAPL antibody
Anti-MTS1 antibody

Information sourced from Uniprot.org

Citations

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