Anti-RYR2 antibody (4932-4948) (STJ25428)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RYR2 (4932-4948) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:100-1:500IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RYR2 |
| Gene ID | 6262 |
| Uniprot ID | RYR2_HUMAN |
| Immunogen Region | 4932-4948 |
| Immunogen Sequence | DAFGELRDQQEQVKEDMETK CFICGIGNDYFDTVPHGFET HTLQEHNLANYLFFLMYLIN KDETEHTGQESYVWKMYQER CWEFFPAGDCFRKQYEDQLN |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 4868-4967 of human RYR2 (NP_001026.2). |
Additional Info
| Tissue Specificity | Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta. |
| Post Translational Modifications | Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels. Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium. |
| Function | Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) cytosolic levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development. |
| Protein Name | Ryanodine Receptor 2Ryr-2Ryr2Hryr-2Cardiac Muscle Ryanodine ReceptorCardiac Muscle Ryanodine Receptor-Calcium Release ChannelType 2 Ryanodine Receptor |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-5578775 |
| Cellular Localisation | Sarcoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Ryanodine Receptor 2 antibodyAnti-Ryr-2 antibodyAnti-Ryr2 antibodyAnti-Hryr-2 antibodyAnti-Cardiac Muscle Ryanodine Receptor antibodyAnti-Cardiac Muscle Ryanodine Receptor-Calcium Release Channel antibodyAnti-Type 2 Ryanodine Receptor antibodyAnti-RYR2 antibody |
Information sourced from Uniprot.org