| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-TIP49A (350-456) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200ChIP 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RUVBL1 |
| Gene ID: |
8607 |
| Uniprot ID: |
RUVB1_HUMAN |
| Immunogen Region: |
350-456 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 350-456 of human RUVBL1/TIP49A/PONTIN (Q9Y265). |
| Immunogen Sequence: |
IPLDLLDRVMIIRTMLYTPQ EMKQIIKIRAQTEGINISEE ALNHLGEIGTKTTLRYSVQL LTPANLLAKINGKDSIEKEH VEEISELFYDAKSSAKILAD QQDKYMK |
| Tissue Specificity | Ubiquitously expressed with high expression in heart, skeletal muscle and testis. |
| Function | Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity.hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation. |
| Protein Name | Ruvb-Like 149 Kda Tata Box-Binding Protein-Interacting Protein49 Kda Tbp-Interacting Protein54 Kda Erythrocyte Cytosolic ProteinEcp-54Ino80 Complex Subunit HNuclear Matrix Protein 238Nmp 238Pontin 52Tip49aTip60-Associated Protein 54-AlphaTap54-Alpha |
| Database Links | Reactome: R-HSA-171319Reactome: R-HSA-201722Reactome: R-HSA-3214847Reactome: R-HSA-5689603Reactome: R-HSA-5689880Reactome: R-HSA-5696394Reactome: R-HSA-606279 |
| Cellular Localisation | Nucleus MatrixNucleusNucleoplasmCytoplasmMembraneCytoskeletonMicrotubule Organizing CenterCentrosomeDynein Axonemal ParticleMainly Localized In The NucleusAssociated With Nuclear Matrix Or In The Nuclear CytosolAlthough It Is Also Present In The Cytoplasm And Associated With The Cell MembranesIn Prophase And Prometaphase It Is Located At The Centrosome And The Branching Microtubule SpindlesAfter Mitotic Nuclear Membrane Disintigration It Accumulates At The Centrosome And Sites Of Tubulin PolymerizationAs Cells Pass Through Metaphase And Into Telophase It Is Located Close To The Centrosome At The Early Phase Of Tubulin PolymerizationIn Anaphase It Accumulates At The Zone Of Tubule InterdigitationIn Telophase It Is Found At Polar Tubule OverlapAnd It Reappears At The Site Of Chromosomal Decondensation In The Daughter Cells |
| Alternative Antibody Names | Anti-Ruvb-Like 1 antibodyAnti-49 Kda Tata Box-Binding Protein-Interacting Protein antibodyAnti-49 Kda Tbp-Interacting Protein antibodyAnti-54 Kda Erythrocyte Cytosolic Protein antibodyAnti-Ecp-54 antibodyAnti-Ino80 Complex Subunit H antibodyAnti-Nuclear Matrix Protein 238 antibodyAnti-Nmp 238 antibodyAnti-Pontin 52 antibodyAnti-Tip49a antibodyAnti-Tip60-Associated Protein 54-Alpha antibodyAnti-Tap54-Alpha antibodyAnti-RUVBL1 antibodyAnti-INO80H antibodyAnti-NMP238 antibodyAnti-TIP49 antibodyAnti-TIP49A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
