Anti-Runx3 antibody (Internal Region) (STJ13100300)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the internal region of rat Runx3 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse.
General Information
| Short Description | Rabbit polyclonal anti-Runx3 (Internal Region) for use in IHC and WB in Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Runx3 |
| Gene ID | 12399 |
| Uniprot ID | RUNX3_MOUSE |
| Immunogen | A synthetic peptide from the internal region of rat Runx3 conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse. |
| Immunogen Region | Internal Region |
| Specificity | Specific for Runx3. |
Additional Info
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. Necessary for the development and survival of sensory neurons expressing parvalbumin. |
| Protein Name | Runt-Related Transcription Factor 3Acute Myeloid Leukemia 2 ProteinCore-Binding Factor Subunit Alpha-3Cbf-Alpha-3Oncogene Aml-2Polyomavirus Enhancer-Binding Protein 2 Alpha C SubunitPea2-Alpha CPebp2-Alpha CSl3-3 Enhancer Factor 1 Alpha C SubunitSl3/Akv Core-Binding Factor Alpha C Subunit |
| Database Links | Reactome: R-MMU-8941855Reactome: -MMU-8941856Reactome: -MMU-8941858Reactome: -MMU-8951430Reactome: -MMU-8951671Reactome: -MMU-8951936 |
| Cellular Localisation | NucleusCytoplasmThe Tyrosine Phosphorylated Form Localizes To The CytoplasmTranslocates From The Cytoplasm To The Nucleus Following Tgf-Beta Stimulation |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 3 antibodyAnti-Acute Myeloid Leukemia 2 Protein antibodyAnti-Core-Binding Factor Subunit Alpha-3 antibodyAnti-Cbf-Alpha-3 antibodyAnti-Oncogene Aml-2 antibodyAnti-Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit antibodyAnti-Pea2-Alpha C antibodyAnti-Pebp2-Alpha C antibodyAnti-Sl3-3 Enhancer Factor 1 Alpha C Subunit antibodyAnti-Sl3/Akv Core-Binding Factor Alpha C Subunit antibodyAnti-Runx3 antibodyAnti-Aml2 antibodyAnti-Cbfa3 antibodyAnti-Pebp2a3 antibody |
Information sourced from Uniprot.org