| Host: | Goat |
| Applications: | IHC/WB |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Runx3 (380-415 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | RUNX3 |
| Gene ID: | 864 |
| Uniprot ID: | RUNX3_HUMAN |
| Immunogen Region: | 380-415 aa |
| Specificity: | Specific for Runx3. |
| Immunogen: | A synthetic peptide from aa region 380-415 of human Runx3 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing. |
| Protein Name | Runt-Related Transcription Factor 3 Acute Myeloid Leukemia 2 Protein Core-Binding Factor Subunit Alpha-3 Cbf-Alpha-3 Oncogene Aml-2 Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit Pea2-Alpha C Pebp2-Alpha C Sl3-3 Enhancer Factor 1 Alpha C Subunit Sl3/Akv Core-Binding Factor Alpha C Subunit |
| Database Links | Reactome: R-HSA-4411364 Reactome: R-HSA-8941855 Reactome: R-HSA-8941856 Reactome: R-HSA-8941858 Reactome: R-HSA-8949275 Reactome: R-HSA-8951430 Reactome: R-HSA-8951671 Reactome: R-HSA-8951911 Reactome: R-HSA-8951936 Reactome: R-HSA-8952158 |
| Cellular Localisation | Nucleus Cytoplasm The Tyrosine Phosphorylated Form Localizes To The Cytoplasm Translocates From The Cytoplasm To The Nucleus Following Tgf-Beta Stimulation |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 3 antibody Anti-Acute Myeloid Leukemia 2 Protein antibody Anti-Core-Binding Factor Subunit Alpha-3 antibody Anti-Cbf-Alpha-3 antibody Anti-Oncogene Aml-2 antibody Anti-Polyomavirus Enhancer-Binding Protein 2 Alpha C Subunit antibody Anti-Pea2-Alpha C antibody Anti-Pebp2-Alpha C antibody Anti-Sl3-3 Enhancer Factor 1 Alpha C Subunit antibody Anti-Sl3/Akv Core-Binding Factor Alpha C Subunit antibody Anti-RUNX3 antibody Anti-AML2 antibody Anti-CBFA3 antibody Anti-PEBP2A3 antibody |
Information sourced from Uniprot.org