| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RUNX2 (242-521) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RUNX2 |
| Gene ID: |
860 |
| Uniprot ID: |
RUNX2_HUMAN |
| Immunogen Region: |
242-521 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 242-521 of human RUNX2 (NP_001019801.3). |
| Immunogen Sequence: |
FSDRLSDLGRIPHPSMRVGV PPQNPRPSLNSAPSPFNPQG QSQITDPRQAQSSPPWSYDQ SYPSYLSQMTSPSIHSTTPL SSTRGTGLPAITDVPRRISD DDTATSDFCLWPSTLSKKSQ AGASELGPFSDPRQFPSISS LTESRFSNPRMHYPATFTYT PPVTSGMSLGMSATTHYHTY LPPPYPGSSQSQSGPFQTSS TPYLYYGTSSGSYQFPMVPG GDRSPSRMLPPCTTTSNGS |
| Tissue Specificity | Specifically expressed in osteoblasts. |
| Post Translational Modifications | Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-451 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. Isoform 3 is phosphorylated on Ser-340. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. |
| Protein Name | Runt-Related Transcription Factor 2Acute Myeloid Leukemia 3 ProteinCore-Binding Factor Subunit Alpha-1Cbf-Alpha-1Oncogene Aml-3Osteoblast-Specific Transcription Factor 2Osf-2Polyomavirus Enhancer-Binding Protein 2 Alpha A SubunitPea2-Alpha APebp2-Alpha ASl3-3 Enhancer Factor 1 Alpha A SubunitSl3/Akv Core-Binding Factor Alpha A Subunit |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-8878166Reactome: R-HSA-8939246Reactome: R-HSA-8939902Reactome: R-HSA-8940973Reactome: R-HSA-8941284Reactome: R-HSA-8941326Reactome: R-HSA-8941332Reactome: R-HSA-8941333 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Runt-Related Transcription Factor 2 antibodyAnti-Acute Myeloid Leukemia 3 Protein antibodyAnti-Core-Binding Factor Subunit Alpha-1 antibodyAnti-Cbf-Alpha-1 antibodyAnti-Oncogene Aml-3 antibodyAnti-Osteoblast-Specific Transcription Factor 2 antibodyAnti-Osf-2 antibodyAnti-Polyomavirus Enhancer-Binding Protein 2 Alpha A Subunit antibodyAnti-Pea2-Alpha A antibodyAnti-Pebp2-Alpha A antibodyAnti-Sl3-3 Enhancer Factor 1 Alpha A Subunit antibodyAnti-Sl3/Akv Core-Binding Factor Alpha A Subunit antibodyAnti-RUNX2 antibodyAnti-AML3 antibodyAnti-CBFA1 antibodyAnti-OSF2 antibodyAnti-PEBP2A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
