| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rubicon (1-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RUBCN |
| Gene ID: |
9711 |
| Uniprot ID: |
RUBIC_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Rubicon (NP_055502.1). |
| Immunogen Sequence: |
MRPEGAGMELGGGEERLPEE SRREHWQLLGNLKTTVEGLV STNSPNVWSKYGGLERLCRD MQSILYHGLIRDQACRRQTD YWQFVKDIRWLSPHSALHVE KFISVHENDQSSADGASERA VAELWLQHSLQYHCLSAQLR PLLGDRQYIRKFYTDAAFLL SDAHVTAMLQCLEAVEQNNP RLLAQIDASMFARKHESPLL |
| Function | Inhibits PIK3C3 activity.under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation. Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes. |
| Protein Name | Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing ProteinRubiconBeclin-1 Associated Run Domain Containing ProteinBaron |
| Cellular Localisation | Late EndosomeLysosomeEarly EndosomePredominantly Located In Late Endosomes/LysosomesOnly Partially Detected In Early Endosome And Not At All In The Golgi Apparatus |
| Alternative Antibody Names | Anti-Run Domain Beclin-1-Interacting And Cysteine-Rich Domain-Containing Protein antibodyAnti-Rubicon antibodyAnti-Beclin-1 Associated Run Domain Containing Protein antibodyAnti-Baron antibodyAnti-RUBCN antibodyAnti-KIAA0226 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
