• Western blot analysis of extracts of various cell lines, using RTCB Antibody (STJ112343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RTCB antibody (236-505) (STJ112343)

SKU:
STJ112343

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RTCB (236-505) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RTCB
Gene ID: 51493
Uniprot ID: RTCB_HUMAN
Immunogen Region: 236-505
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 236-505 of human RTCB (NP_055121.1).
Immunogen Sequence: EIFNEYAAKKMGIDHKGQVC VMIHSGSRGLGHQVATDALV AMEKAMKRDKIIVNDRQLAC ARIASPEGQDYLKGMAAAGN YAWVNRSSMTFLTRQAFAKV FNTTPDDLDLHVIYDVSHNI AKVEQHVVDGKERTLLVHRK GSTRAFPPHHPLIAVDYQLT GQPVLIGGTMGTCSYVLTGT EQGMTETFGTTCHGAGRALS RAKSRRNLDFQDVLDKLADM GIAIRVASPKLVMEEAPES
Function Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Protein Name Rna-Splicing Ligase Rtcb Homolog
3'-Phosphate/5'-Hydroxy Nucleic Acid Ligase
Database Links Reactome: R-HSA-6784531
Cellular Localisation Nucleus
Cytoplasm
Enters Into The Nucleus In Case Of Active Transcription While It Accumulates In Cytosol When Transcription Level Is Low
Alternative Antibody Names Anti-Rna-Splicing Ligase Rtcb Homolog antibody
Anti-3'-Phosphate/5'-Hydroxy Nucleic Acid Ligase antibody
Anti-RTCB antibody
Anti-C22orf28 antibody
Anti-HSPC117 antibody

Information sourced from Uniprot.org

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