• Immunofluorescence analysis of MCF-7 cells using RSRC1 antibody (STJ29290).
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RSRC1 antibody (STJ29290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using RSRC1 antibody (STJ29290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using RSRC1 antibody (STJ29290) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RSRC1 antibody (STJ29290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RSRC1 antibody (117-276) (STJ29290)

SKU:
STJ29290

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RSRC1 (117-276) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSRC1
Gene ID: 51319
Uniprot ID: RSRC1_HUMAN
Immunogen Region: 117-276
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 117-276 of human RSRC1 (NP_001258763.1).
Immunogen Sequence: EHLPPAEQAKARLQLVLEAA AKADEALKAKERNEEEAKRR KEEDQATLVEQVKRVKEIEA IESDSFVQQTFRSSKEVKKS VEPSEVKQATSTSGPASAVA DPPSTEKEIDPTSIPTAIKY QDDNSLAHPNLFIEKADAEE KWFKRLIALRQERLMGSPVA
Tissue Specificity Widely expressed. Expressed in brain, spinal cord, cerebellum.
Post Translational Modifications Phosphorylated.
Function Has a role in alternative splicing and transcription regulation. Involved in both constitutive and alternative pre-mRNA splicing. May have a role in the recognition of the 3' splice site during the second step of splicing.
Protein Name Serine/Arginine-Related Protein 53
Srrp53
Arginine/Serine-Rich Coiled-Coil Protein 1
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm
Alternative Antibody Names Anti-Serine/Arginine-Related Protein 53 antibody
Anti-Srrp53 antibody
Anti-Arginine/Serine-Rich Coiled-Coil Protein 1 antibody
Anti-RSRC1 antibody
Anti-SRRP53 antibody
Anti-BM-011 antibody

Information sourced from Uniprot.org

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