• Western blot analysis of extracts of various cell lines, using RSPH9 antibody (STJ118417) at 1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RSPH9 antibody (1-276) (STJ118417)

SKU:
STJ118417

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RSPH9 (1-276) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RSPH9
Gene ID: 221421
Uniprot ID: RSPH9_HUMAN
Immunogen Region: 1-276
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human RSPH9 (NP_689945.2).
Immunogen Sequence: MDADSLLLSLELASGSGQGL SPDRRASLLTSLMLVKRDYR YDRVLFWGRILGLVADYYIA QGLSEDQLAPRKTLYSLNCT EWSLLPPATEEMVAQSSVVK GRFMGDPSYEYEHTELQKVN EGEKVFEEEIVVQIKEETRL VSVIDQIDKAVAIIPRGALF KTPFGPTHVNRTFEGLSLSE AKKLSSYFHFREPVELKNKT LLEKADLDPSLDFMDSLEHD IPKGSWSIQMERGNALVVL
Function Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia. Essential for both the radial spoke head assembly and the central pair microtubule stability in ependymal motile cilia. Required for motility of olfactory and neural cilia and for the structural integrity of ciliary axonemes in both 9+0 and 9+2 motile cilia.
Protein Name Radial Spoke Head Protein 9 Homolog
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Axoneme
Flagellum Axoneme
Cell Projection
Kinocilium
Alternative Antibody Names Anti-Radial Spoke Head Protein 9 Homolog antibody
Anti-RSPH9 antibody
Anti-C6orf206 antibody
Anti-MRPS18AL1 antibody

Information sourced from Uniprot.org

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