| Short Description : | Rabbit polyclonal RRP8 (100-200) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RRP8 |
| Gene ID: | 23378 |
| Uniprot ID: | RRP8_HUMAN |
| Immunogen Region: | 100-200 |
| Immunogen Sequence: | GPPCSDSEEEVERKKKCHKQ ALVGSDSAEDEKRKRKCQKH APINSAQHLDNVDQTGPKAW KGSTTNDPPKQSPGSTSPKP PHTLSRKQWRNRQKNKRRCK N |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RRP8 (NP_056139.1). |
| Function | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. |
| Protein Name | Ribosomal Rna-Processing Protein 8Cerebral Protein 1Nucleomethylin |
| Database Links | Reactome: R-HSA-427359 |
| Cellular Localisation | NucleusNucleolusLocalizes At Rdna Locus |
| Alternative Antibody Names | Anti-Ribosomal Rna-Processing Protein 8 antibodyAnti-Cerebral Protein 1 antibodyAnti-Nucleomethylin antibodyAnti-RRP8 antibodyAnti-KIAA0409 antibodyAnti-NML antibodyAnti-hucep-1 antibody |
Information sourced from Uniprot.org
