• Western blot analysis of extracts of various cell lines, using RRM2 Rabbit monoclonal antibody (STJ11101866) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RRM2 antibody (10-117) [S6MR] (STJ11101866)

SKU:
STJ11101866

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RRM2 (10-117) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRM2
Gene ID: 6241
Uniprot ID: RIR2_HUMAN
Immunogen Region: 10-117
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-117 of human RRM2 (P31350).
Immunogen Sequence: PITDPQQLQLSPLKGLSLVD KENTPPALSGTRVLASKTAR RIFQEPTEPKTKAAAPGVED EPLLRENPRRFVIFPIEYHD IWQMYKKAEASFWTAEEVDL SKDIQHWE
Post Translational Modifications Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. Phosphorylated on Thr-33 by CDK1 and CDK2.predominantly in G2 and M phase. Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.leading to its degradation by the proteasome.
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Protein Name Ribonucleoside-Diphosphate Reductase Subunit M2
Ribonucleotide Reductase Small Chain
Ribonucleotide Reductase Small Subunit
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-69205
Reactome: R-HSA-8953750
Cellular Localisation Cytoplasm
Nucleus
Localized To The Cytoplasm In S Phase Cells
May Localize To The Nucleus In G2 Phase Cells
Alternative Antibody Names Anti-Ribonucleoside-Diphosphate Reductase Subunit M2 antibody
Anti-Ribonucleotide Reductase Small Chain antibody
Anti-Ribonucleotide Reductase Small Subunit antibody
Anti-RRM2 antibody
Anti-RR2 antibody

Information sourced from Uniprot.org

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