• Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells using 3 Mu g RRM1 antibody (STJ25417). Western blot was performed from the immunoprecipitate using RRM1 antibody (STJ25417) at a dilution of 1:1000.
  • Immunofluorescence analysis of HeLa cells using RRM1 Rabbit polyclonal antibody (STJ25417) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RRM1 in paraffin-embedded human colon using RRM1 Rabbit polyclonal antibody (STJ25417) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat lung, using RRM1 Rabbit polyclonal antibody (STJ25417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of various lysates using RRM1 Rabbit polyclonal antibody (STJ25417) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RRM1 antibody (593-792) (STJ25417)

SKU:
STJ25417

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRM1 (593-792) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRM1
Gene ID: 6240
Uniprot ID: RIR1_HUMAN
Immunogen Region: 593-792
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).
Immunogen Sequence: IRNSLLIAPMPTASTAQILG NNESIEPYTSNIYTRRVLSG EFQIVNPHLLKDLTERGLWH EEMKNQIIACNGSIQSIPEI PDDLKQLYKTVWEISQKTVL KMAAERGAFIDQSQSLNIHI AEPNYGKLTSMHFYGWKQGL KTGMYYLRTRPAANPIQFTL NKEKLKDKEKVSKEEEEKER NTAAMVCSLENRDECLMCGS
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Protein Name Ribonucleoside-Diphosphate Reductase Large Subunit
Ribonucleoside-Diphosphate Reductase Subunit M1
Ribonucleotide Reductase Large Subunit
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ribonucleoside-Diphosphate Reductase Large Subunit antibody
Anti-Ribonucleoside-Diphosphate Reductase Subunit M1 antibody
Anti-Ribonucleotide Reductase Large Subunit antibody
Anti-RRM1 antibody
Anti-RR1 antibody

Information sourced from Uniprot.org

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