• Western blot analysis of extracts of various cell lines, using RRM1 antibody (STJ115311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Western blot analysis of extracts of various cell lines, using RRM1 antibody (STJ115311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST.
  • Immunofluorescence analysis of HeLa cells using RRM1 rabbit polyclonal antibody (STJ115311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using RRM1 rabbit polyclonal antibody (STJ115311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g RRM1 antibody (STJ115311). Western blot was performed from the immunoprecipitate using RRM1 antibody (STJ115311) at a dilution of 1:500.

Anti-RRM1 antibody (593-792) (STJ115311)

SKU:
STJ115311

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRM1 (593-792) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRM1
Gene ID: 6240
Uniprot ID: RIR1_HUMAN
Immunogen Region: 593-792
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).
Immunogen Sequence: IRNSLLIAPMPTASTAQILG NNESIEPYTSNIYTRRVLSG EFQIVNPHLLKDLTERGLWH EEMKNQIIACNGSIQSIPEI PDDLKQLYKTVWEISQKTVL KMAAERGAFIDQSQSLNIHI AEPNYGKLTSMHFYGWKQGL KTGMYYLRTRPAANPIQFTL NKEKLKDKEKVSKEEEEKER NTAAMVCSLENRDECLMCGS
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Protein Name Ribonucleoside-Diphosphate Reductase Large Subunit
Ribonucleoside-Diphosphate Reductase Subunit M1
Ribonucleotide Reductase Large Subunit
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Ribonucleoside-Diphosphate Reductase Large Subunit antibody
Anti-Ribonucleoside-Diphosphate Reductase Subunit M1 antibody
Anti-Ribonucleotide Reductase Large Subunit antibody
Anti-RRM1 antibody
Anti-RR1 antibody

Information sourced from Uniprot.org

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