| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RRAGA (213-313) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RRAGA |
| Gene ID: |
10670 |
| Uniprot ID: |
RRAGA_HUMAN |
| Immunogen Region: |
213-313 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 213-313 of human RRAGA (NP_006561.1). |
| Immunogen Sequence: |
VISHYQCKEQRDVHRFEKIS NIIKQFKLSCSKLAASFQSM EVRNSNFAAFIDIFTSNTYV MVVMSDPSIPSAATLINIRN ARKHFEKLERVDGPKHSLLM R |
| Tissue Specificity | Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain. |
| Post Translational Modifications | Polybiquitinated via 'Lys-63'-linked polyubiquitination by RNF152 in response to amino acid starvation: polyubiquitination of the GDP-bound inactive form by RNF152 promotes RRAGA inactivation and interaction with the GATOR1 complex. This does not affect RRAGA degradation. |
| Function | Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form: RagA/RRAGA is in its active form when GTP-bound RagA/RRAGA forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagA/RRAGA heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. |
| Protein Name | Ras-Related Gtp-Binding Protein ARag ARagaAdenovirus E3 14.7 Kda-Interacting Protein 1Fip-1 |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8866654Reactome: R-HSA-8943724Reactome: R-HSA-9639288 |
| Cellular Localisation | CytoplasmNucleusLysosome MembranePredominantly CytoplasmicRecruited To The Lysosome Surface By The Ragulator ComplexMay Shuttle Between The Cytoplasm And NucleusDepending On The Bound Nucleotide StateColocalizes In Vivo With Adenovirus E3-147k Mainly To The Cytoplasm Especially Near The Nuclear Membrane And In Discrete Foci On Or Near The Plasma Membrane |
| Alternative Antibody Names | Anti-Ras-Related Gtp-Binding Protein A antibodyAnti-Rag A antibodyAnti-Raga antibodyAnti-Adenovirus E3 14.7 Kda-Interacting Protein 1 antibodyAnti-Fip-1 antibodyAnti-RRAGA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
