• Western blot analysis of extracts of mouse brain, using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Confocal imaging of U-2 OS cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat ovary using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at dilution of 1:100 (40x lens).
  • Confocal imaging of NIH/3T3 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human colon using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at dilution of 1:100 (40x lens).
  • Confocal imaging of C2C12 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded mouse kidney using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded mouse kidney tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of U-2 OS cells using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded rat colon tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RPS6KB2 antibody (1-120) [S3MR] (STJ11102423)

SKU:
STJ11102423

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-P70 S6 Kinase beta (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS6KB2
Gene ID: 6199
Uniprot ID: KS6B2_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human p70 S6 Kinase 2 (Q9UBS0).
Immunogen Sequence: MAAVFDLDLETEEGSEGEGE PELSPADACPLAELRAAGLE PVGHYEEVELTETSVNVGPE RIGPHCFELLRVLGKGGYGK VFQVRKVQGTNLGKIYAMKV LRKAKIVRNAKDTAHTRAER
Post Translational Modifications Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.
Function Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.
Protein Name Ribosomal Protein S6 Kinase Beta-2
S6k-Beta-2
S6k2
70 Kda Ribosomal Protein S6 Kinase 2
P70s6k2
P70-S6k 2
S6 Kinase-Related Kinase
Srk
Serine/Threonine-Protein Kinase 14b
P70 Ribosomal S6 Kinase Beta
S6k-Beta
P70 S6 Kinase Beta
P70 S6k-Beta
P70 S6kb
P70-Beta
Database Links Reactome: R-HSA-198693
Reactome: R-HSA-5674400
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Ribosomal Protein S6 Kinase Beta-2 antibody
Anti-S6k-Beta-2 antibody
Anti-S6k2 antibody
Anti-70 Kda Ribosomal Protein S6 Kinase 2 antibody
Anti-P70s6k2 antibody
Anti-P70-S6k 2 antibody
Anti-S6 Kinase-Related Kinase antibody
Anti-Srk antibody
Anti-Serine/Threonine-Protein Kinase 14b antibody
Anti-P70 Ribosomal S6 Kinase Beta antibody
Anti-S6k-Beta antibody
Anti-P70 S6 Kinase Beta antibody
Anti-P70 S6k-Beta antibody
Anti-P70 S6kb antibody
Anti-P70-Beta antibody
Anti-RPS6KB2 antibody
Anti-STK14B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance