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Anti-RPS6KB2 antibody (1-120) [S3MR] (STJ11102423)

Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of mouse brain, using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Confocal imaging of U-2 OS cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of C2C12 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of mouse brain, using p70 S6 Kinase 2 rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Confocal imaging of U-2 OS cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of C2C12 cells using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human cervix cancer tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Immunohistochemistry analysis of p70 S6 Kinase 2 in paraffin-embedded human colon carcinoma tissue using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Western blot analysis of lysates from Mouse brain, using p70 S6 Kinase 2 Rabbit monoclonal antibody (STJ11102423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RPS6KB2 antibody (1-120) [S3MR] (STJ11102423)

SKU:

STJ11102423

£129.50 - £574.50

Size: Required 20 µL £129.50 100 µL £298.50 200 µL (2 x 100 µL) £574.50

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General Information
Product Properties
Target Information
Additional Info

Host:	Rabbit
Applications:	WB/IHC-P/IF/ICC/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality:	Monoclonal
Clone ID:	S3MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification:	Affinity purification
Concentration:	Lot specific
Dilution Range:	WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	RPS6KB2
Gene ID:	6199
Uniprot ID:	KS6B2_HUMAN
Immunogen Region:	1-120
Specificity:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human p70 S6 Kinase 2 (Q9UBS0).
Immunogen Sequence:	MAAVFDLDLETEEGSEGEGE PELSPADACPLAELRAAGLE PVGHYEEVELTETSVNVGPE RIGPHCFELLRVLGKGGYGK VFQVRKVQGTNLGKIYAMKV LRKAKIVRNAKDTAHTRAER

Post Translational Modifications	Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.
Function	Phosphorylates specifically ribosomal protein S6. Seems to act downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression in an alternative pathway regulated by MEAK7.
Protein Name	Ribosomal Protein S6 Kinase Beta-2 S6k-Beta-2 S6k2 70 Kda Ribosomal Protein S6 Kinase 2 P70s6k2 P70-S6k 2 S6 Kinase-Related Kinase Srk Serine/Threonine-Protein Kinase 14b P70 Ribosomal S6 Kinase Beta S6k-Beta P70 S6 Kinase Beta P70 S6k-Beta P70 S6kb P70-Beta
Database Links	Reactome: R-HSA-198693 Reactome: R-HSA-5674400
Cellular Localisation	Cytoplasm Nucleus
Alternative Antibody Names	Anti-Ribosomal Protein S6 Kinase Beta-2 antibody Anti-S6k-Beta-2 antibody Anti-S6k2 antibody Anti-70 Kda Ribosomal Protein S6 Kinase 2 antibody Anti-P70s6k2 antibody Anti-P70-S6k 2 antibody Anti-S6 Kinase-Related Kinase antibody Anti-Srk antibody Anti-Serine/Threonine-Protein Kinase 14b antibody Anti-P70 Ribosomal S6 Kinase Beta antibody Anti-S6k-Beta antibody Anti-P70 S6 Kinase Beta antibody Anti-P70 S6k-Beta antibody Anti-P70 S6kb antibody Anti-P70-Beta antibody Anti-RPS6KB2 antibody Anti-STK14B antibody

Information sourced from Uniprot.org

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