• Western blot analysis of extracts of various cell lines, using RPP25 antibody (STJ112014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPP25 Rabbit polyclonal antibody (STJ112014) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon using RPP25 rabbit polyclonal antibody (STJ112014) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon using RPP25 Rabbit polyclonal antibody (STJ112014) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using RPP25 rabbit polyclonal antibody (STJ112014) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using RPP25 antibody (STJ112014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RPP25 antibody (1-199) (STJ112014)

SKU:
STJ112014

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPP25 (1-199) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPP25
Gene ID: 54913
Uniprot ID: RPP25_HUMAN
Immunogen Region: 1-199
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RPP25 (NP_060263.2).
Immunogen Sequence: MENFRKVRSEEAPAGCGAEG GGPGSGPFADLAPGAVHMRV KEGSKIRNLMAFATASMAQP ATRAIVFSGCGRATTKTVTC AEILKRRLAGLHQVTRLRYR SVREVWQSLPPGPTQGQTPG EPAASLSVLKNVPGLAILLS KDALDPRQPGYQPPNPHPGP SSPPAAPASKRSLGEPAAGE GSAKRSQPEPGVADEDQTA
Function Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.
Protein Name Ribonuclease P Protein Subunit P25
Rnase P Protein Subunit P25
Database Links Reactome: R-HSA-6784531
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribonuclease P Protein Subunit P25 antibody
Anti-Rnase P Protein Subunit P25 antibody
Anti-RPP25 antibody

Information sourced from Uniprot.org

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